Cortisol is also known as hydrocortisone when used medically, is usually a supplement or for anti-inflammatory properties. Kortisol Kortisol Kortisol is useful in identifying a serious lack of cortisol usually found in Addison disease, or serious cortisol production as in cushing syndrome. Cortisol analysis is also often used to evaluate the level of stress of a subject.
Elisa is designed to detect cortisol in biological fluid including urine, plasma, and saliva. This kit is a competitive immunoassay enzyme (Elisa) to determine cortisol levels in biological samples including urine, saliva, and plasma. You can know more about Cortisol ELISA at Boster Bio.
Briefly, cortisol in samples or standards competes with conjugated cortisol to radish peroxidase (HRP) to bind to polyclonal antibodies specifically for cortisol coated with microplates. HRP activity produces brilliant blue development when the substrate is added, with the intensity of color development proportional to the number of cortisol present.
When measuring urine samples, it is recommended to normalize samples to creatinine to take into account the variations of fluid intake and excretion. Creatinine is announced at a constant level and can be used to normalize the pace of other analytics excretion. This kit is of competitive format.
Human cortisol Elisa measures cortisol in serum human, plasma, urine, saliva, dried stool extract or or cell culture media. Elisa-use kit ELISA competitive cortisol is designed to measure cortisol quantitatively in dried stool extract, saliva, urine, serum, plasma, and sample media culture media. The total cortisol is measured in extracted samples and in serum and plasma, and free cortisol in saliva and urine.
Cortisol standards are provided to produce a standard curve for tests and all samples are read from the standard user-generated curve. Standards or samples diluted pipettes into the microtiter plates are clearly coated with antibodies to capture mouse antibodies.
Conjugation of cortisol-peroxidase is added to the well. A binding reaction was initiated by the addition of monoclonal antibodies to cortisol. After 1 hour incubation, the dish was washed and the substrate was added. The substrate reacts with bound cortisol-peroxidase conjugates. After short incubation, the reaction is stopped and the intensity of the resulting color is detected in the microtiter plate reader at 450 nm.